Coding

Part:BBa_K3982000:Design

Designed by: Akankshya Ramkrishna Sahu   Group: iGEM21_IISER_Berhampur   (2021-09-29)


6xHis - Tagged Cas14a1 with TEV site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2047
    Illegal EcoRI site found at 3487
    Illegal XbaI site found at 2062
    Illegal PstI site found at 14
    Illegal PstI site found at 2277
    Illegal PstI site found at 2443
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2047
    Illegal EcoRI site found at 3487
    Illegal PstI site found at 14
    Illegal PstI site found at 2277
    Illegal PstI site found at 2443
    Illegal NotI site found at 7
    Illegal NotI site found at 2053
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2047
    Illegal EcoRI site found at 3487
    Illegal BamHI site found at 3081
    Illegal XhoI site found at 1031
    Illegal XhoI site found at 1923
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2047
    Illegal EcoRI site found at 3487
    Illegal PstI site found at 14
    Illegal PstI site found at 2277
    Illegal PstI site found at 2443
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2047
    Illegal EcoRI site found at 3487
    Illegal XbaI site found at 2062
    Illegal PstI site found at 14
    Illegal PstI site found at 2277
    Illegal PstI site found at 2443
    Illegal AgeI site found at 2737
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Restriction cloning was done to clone this sequence in the pSB1C3 backbone using Xba1 and Spe1 Restriction enzymes.


Source

The sequence of this part is derived from Addgene plasmid number 112502

References